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. 2021 Apr 24;13(5):1448. doi: 10.3390/nu13051448

Figure 6.

Figure 6

Effect of Gly-Sar on the transport of γ-EV across Caco-2 monolayer cells. (A) The transport of 1 mM γ-EV, assessed over a period of 2 h, after the pretreatment of Gly-Sar (25 mM, 30 min). (B) Immunofluorescence staining of the tight-junction proteins ZO-1 and claudin-1 of the Gly-Sar-treated Caco-2 monolayer cells on the membrane, to ensure the maintenance of cell integrity after 2 h of transport. (C) The transport of 1 mM γ-EV, assessed over a period of 2 h, after the pre-treatment of both TF3’G and Gly-Sar simultaneously. Control immunofluorescence images are same as those mentioned in Figure 4. Data presented as the mean ± SD from at least n = 3 (Gly-Sar) and n = 4 (control and TF3′G/Gly-Sar) independent experiments; * and ** indicate p < 0.05 and p < 0.01, respectively.