Figure 3.

Centrifugation of calcein-loaded PDLCs. (A) Schematic figure of centrifugal forces applied to each particle during centrifugation. The magnitude of the centrifugal force has a direct relation with the mass of the particle. (B) Samples after centrifugation and removal of the supernatant. The absence of conjugation in DBCO blocked droplets causes the washing of the unbound liposomes. The orange color of the pellet in the PDLC sample is indicative of the existence of quenched calcein in the liposomes that are bound to droplets and retained in the pellet. (C) Percent of droplets retained in the pellet after three centrifugations, as determined by reduction in DiD intensity. Droplets in the PDLC sample carry liposomes and are consequently heavier so they experience higher centrifugal forces. The difference is statistically significant (p < 0.002) and is another indication of tethering droplets to liposomes.