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Phase-contrast images of ARS staining of both vector-transfected (ctrL) and ALDH2 knockdown transfected osteoblasts (ALDH2-shRNA) after culturing in differentiation medium for 7 days and 14 days. Pink staining of Alizarin Red S (ARS) indicates the degree of calcification of the osteoblasts. Left panels represent cells transfected by the control vector on Day 7 and Day 14. Right panels represent cells transfected by the ALDH2-shRNA vector on Day 7 and Day 14.
