Figure 2.

The H₂O₂ -mediated upregulation of Ca²⁺ fluorescence intensity through the stimulation of TRPM2 in the ARPE19. (n = 25–30). After incubating the ARPE19 cells with Fluo-3AM (1 µM for 60 min), the TRPM2 stimulator (H₂O₂ and 1 mM) and blocker (ACA and 25 µM for 6 min) applied to the cells within three groups (control, H₂O₂ and H₂O₂ + ACA). The cells were analyzed. The fluorescence intensity of Fluo-3AM in the ARPE19 was imaged at 515 nm in the LSM 800 laser scan microscope with 40 × 1.3 oil objective and the results were indicated as arbitrary unit (a.u.). (a) Imagines of the Fluo-3AM. (b,c) The mean fluorescence intensity of Ca²⁺ in the column and line figures were expressed as Mean ± SD in the ARPE19 after the treatments of H₂O₂ and ACA. (* p ≤ 0.05 vs. control. ^x p ≤ 0.05 vs. H₂O₂ group).