Figure 4.

The stimulation of H₂O₂ did not induce changes on the [Ca²⁺]_c and TRPM2 activation in the cells of ARPE19-KO. (Mean ± SD). After incubating the cells of ARPE19-KO with Fluo-3AM (1 µM for 60 min), the TRPM2 stimulator (H₂O₂ and 1 mM) and blocker (ACA and 25 µM for 6 min) applied to the cells within three groups (control, H₂O₂ and H₂O₂ + ACA). The images of ARPE19-KO were captured in the LSM 800 with 40 × 1.3 oil objective. The scale bar was kept as 5 µm. The captured representative images (Figure 4a) and the mean Fluo-3AM intensity values (Figure 4b,c) in the groups of control (Cntr), H₂O₂ and H₂O₂ + ACA are shown in the Figure 4. One symbolic image from each group was selected from 25–30 cells of 6 independent investigations.