Figure 1.

Transfection and interference efficiencies of miR-149-5p: (A) qPCR results showing expression levels of bta-miR-149-5p in adipocytes transfected with bta-miR-149-5p mimic and its negative control. (B–D) Transfection efficiency was checked through FAM/labeled miR-NC in bovine adipocytes; after 6-, 12-, and 24-h intervals, the transfection efficiency was measured through an Olympus I × 71 microscope (OLYMPUS) in bovine adipocytes. U6 was used as a house-keeping gene, and the relative expression level in adipocytes transfected with bta-miR-149-5p mimics was normalized with adipocytes transfected with mimic NC. The values presented with mean ± SEM, n = 3, and ** p < 0.01 were considered statistically significant.