Figure 8.

Effect of anti A561L^D4 AB on vLysin activity. PBCV-1 isolated vLysin was treated with anti A561L^D4 antibody (AB) produced in mice (800-fold dilution) for 1 h. After the incubation, anti-mouse Ig MB were added to the reaction and the supernatant fraction was separated from the magnetic beads following the manufacturer’s instructions. Antibody treated vLysin was mixed with NC64A cells and incubated for 1 h. The treated cells were subject to 1% SDS, spun down, the supernatant fraction was removed and chlorophyll release was measured at λ = 420. Untreated PBCV-1 vLysin was used as a positive control. NC64A cells treated with pre-bleed serum served as a negative control. Results are plotted as mean ± standard deviation (n = 4), p-value 0.0001. (*) A mean of standard deviation for vLysin + AB + MB was 0.0002.