Table 4.

Dual plate Interaction of ThSM3a and other fungi.

Fungi	Lifestyle	Plate Interaction	Microscopic
Aspergillus niger	saprophyte	TGI	NR
Colletotrichum gloeosporioides	pathogen	TGI	TMB
Colletorichum magna	pathogen	NI	NR
Curvularia protuberata	mutualist	NI	NR
Curvularia ineaqualis	mutualist	NI	NR
Alternaria alternata	pathogen	TGI	NR
Fusarium culmorum	pathogen	TGI	NR
Penicillium spp.	saprophyte	NI	NR
Fusarium spp.	pathogen	TGI	NR

Fungal Plate interactions: ThSM3a and another saprophytic, mutualistic, or pathogenic fungus (Aspergillus niger, Colletotrichum gloeosporioides, Colletotrichum magna, Curvularia protuberata, Curvularia inaequalis, Alternaria alternata, Fusarium culmorum, Penicillium, or Fusarium spp.) were inoculated onto opposite sides of a 0.1× PDA media plate and fungi allowed to grow within 1 cm of each other and microscopically assessed for the type of interactions observed. The interactions were scored as antagonistic when areas of growth inhibition or branching of mycelia was observed as a result of fungal interaction. When growth was not inhibited and no deviations in mycelia growth observed, these were considered non-inhibitory response. All assays were repeated three times and no standard deviations observed in the interactions. Abbreviations: TGI, growth inhibition of ThSM3a by other fungal species; TGM, mycelial branching of ThSM3a in response to other fungal species; NI, no inhibition of ThSM3a or the other fungal species; NR, no response by ThSM3a or the other fungal species.