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. 2021 Apr 22;9(5):456. doi: 10.3390/biomedicines9050456

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Translation prediction of the wild-type (a) and mutant (b) mRNA. Reading frame 3 is used in the wild-type. Mutant mRNA was predicted to have one out-of-frame and two in-frame upstream open reading frames (denoted by red arrows).