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Genes targeted by conventional PCR and primers used in this study.
| Target | Primer Names | Primer Sequences (5′–3′) | Amplicon Length (bp) | Reference |
|---|---|---|---|---|
| Canine endogenous control (RPS19) | RPS19F RPS19R |
CCTTCCTCAAAAA/GTCTGGG1 GTTCTCATCGTAGGGAGCAAG |
95 | [28] |
| Mycoplasma screening (16S) | Mycop16S rRNA-F Mycop16S rRNA-R |
ATGTTGCTTAATTCGATAATACACGAAA ACRGGATTACTAGTGATTCCAACTTCAA |
384 | [29] |
| Tick endogenous control (16S) | 16S-F 16S-R1 |
TTAAATTGCTGTRGTATT CCGGTCTGAACTCASAWC |
455 | [30] |
