Table 4.
NMR spectroscopic data (500 MHz, D2O), 1H and 13C chemical shifts (δ, ppm) as well as 1H multiplicities of asperulose identified in MeOH40 fraction of G. arenarium.(d: doublet, dd: doublet of doublets, m: multiplet, brs: broad singlet).
| Asperuloside (MeOD, 500 MHz) | ||
|---|---|---|
| Position | TM H | TMC, Type |
| 1 | 5.91 (d) | 95.7, CH |
| 3 | 7.48 (d) | 152.7, CH |
| 4 | - | 107.5, qC |
| 5 | 3.70 (m) | 38.7, CH |
| 6 | 5.68 (dd) | 89.1, CH |
| 7 | 5.77 (d) | 130.9, CH |
| 8 | - | 144.9, qC |
| 9 | 3.38 | 146.2, CH |
| 10 | 4.65/4.78 (m) | 64.3, CH2 |
| 11 | - | 176.2, COOH |
| Ac (CH3) | 2.1 | 23.1, CH3 |
| Ac (C = O) | - | 176.6 |
| 1′ | 4.85 (d) | 103.2, CH |
| 2′ | 3.27 (dd) | 75.5, CH |
| 3′ | 3.51 (m) | 79, CH |
| 4′ | 3.38 (m) | 72.1, CH |
| 5′ | 3.50 (m) | 79.1, CH |
| 6′ | 3.75/3.95 (m) | 63, CH2 |