Table 3.
Target Gene | Type of PCR | Primer Name | Primers/Sond | Amplicon Size (bp) | Ref. |
---|---|---|---|---|---|
OXA-48 | Standard PCR | OXA48_STD_F | TTGGTGGCATCGATTATCGG | 744 | [52] |
OXA48_STD_R | GAGCACTTCTTTTGTGATGGC | ||||
Real-time PCR | OXA48_RT_F | TCTTAAACGGGCGAACCAAG | 125 | [53] | |
OXA48_RT_R | GCGTCTGTCCATCCCACTTA | ||||
OXA48_RT_Probe | 6-FAM-AGCTTGATCGCCCTCGATTTGG-TAMRA | ||||
NDM | Standard PCR | NDM-1-F | CATTTGCGGGGTTTTTAATG | 1022 | [54] |
NDM-1-R | CTGGGTCGAGGTCAGGATAG | ||||
Real-time PCR | NDM_RT_F | GCGCAACACAGCCTGACTTT | 155 | [54] | |
NDM_RT_R | CAGCCACCAAAAGCGATGTC | ||||
NDM_RT_Probe | 6-FAM-CAACCGCGCCCAACTTTGGC-TAMRA | ||||
KPC | Standard PCR | KPC-F | ATGTCACTGTATCGCCGTCT | 893 | [54] |
KPC-R | TTTTCAGAGCCTTACTGCCC | ||||
Real-time PCR | KPC-F | GATACCACGTTCCGTCTGGA | 180 | [23] | |
KPC-R | GGTCGTGTTTCCCTTTAGCC | ||||
KPC-Probe | 6-FAM-CGCGCGCCGTGACGGAAAGC-TAMRA | ||||
VIM | Standard PCR | VIM-F | TGGTCTACATGACCGCGTCT | 766 | [55] |
VIM-R | CGACTGAGCGATTTGTGTG | ||||
Real-time PCR | VIM-F | CACAGYGGCMCTTCTCGCGGAGA | 132 | [53] | |
VIM-R | GCGTACGTYGCCACYCCAGCC | ||||
VIM-Probe | 6-FAM-AGTCTCCACGCACTTTCATGACGACCGCGTCGGCG-TAMRA | ||||
IMP | Standard PCR | IMP-F | CATGGTTTGGTGGTTCTTGT | 488 | [56] |
IMP-R | ATAATTTGGCGGACTTTGGC |