TABLE A7.
List of differential metabolites at the logarithmic phase, stationary phase, and VB12 production phase. log2 (FC) means the log base twofold change values. Differential metabolites were identified using the following criteria: log2 (FC) >1 or <−1, and −log(P) > 1.301 (p‐value < 0.05).
| Logarithmic phase | Stationary phase | VB12 production phase | ||||||
|---|---|---|---|---|---|---|---|---|
| Metabolites | log2 (FC) | ‐lg(P) | Metabolites | log2 (FC) | ‐lg(P) | Metabolites | log2 (FC) | ‐lg(P) |
| 2‐Dehydro‐3‐deoxy‐d‐gluconate | 2.05367 | 4.49607 | 2‐Dehydro−3‐deoxy‐d‐gluconate | −2.51145 | 7.76662 | 6‐Phosphogluconic acid | −3.26569 | 3.17945 |
| 2‐Keto‐gluconic acid | 1.24351 | 2.79056 | Acetyl‐CoA | 3.18808 | 2.65468 | 6‐Phosphogluconic acid | −3.26569 | 3.17945 |
| Acetyl‐CoA | 4.35585 | 2.48321 | Aconitic acid | −1.0933 | 2.46648 | Adenosine monophosphate | 1.89134 | 2.96532 |
| Adenosine monophosphate | 2.37507 | 4.74851 | Adenosine monophosphate | 1.69077 | 1.84774 | Aspartic acid | 1.61263 | 3.45256 |
| Arginine | 3.4152 | 2.49041 | Adenosine triphosphate | 1.23665 | 1.63361 | Aconitic acid | −2.20843 | 4.49006 |
| Aspartic acid | 4.06933 | 4.1856 | Aspartic acid | 1.89534 | 2.28934 | FAD | 1.10727 | 2.00782 |
| cis‐Aconitic acid | 2.47559 | 2.69331 | Citric acid | −2.57374 | 3.50397 | FADH | 1.36122 | 2.59461 |
| Citric acid | 2.10141 | 3.14467 | FAD | 1.10237 | 2.51284 | Fructose 1,6‐bisphosphate | −1.45398 | 2.85088 |
| Cysteine | −3.46801 | 2.38333 | FADH | 1.14113 | 2.36028 | Fumaric acid | −2.1796 | 2.48359 |
| FAD | 1.3632 | 2.03838 | Fructose 1,6‐bisphosphate | −1.56051 | 3.566 | Guanosine monophosphate | −4.57931 | 2.38568 |
| FADH | 2.14206 | 1.81123 | Gluconic acid | −2.54297 | 2.267 | Malic acid | −2.20661 | 2.4959 |
| Fructose 6‐phosphate | 2.40673 | 4.0459 | Gluconolactone | −2.51145 | 7.76662 | Methionine | −1.3884 | 3.28576 |
| Fumaric acid | 2.54599 | 3.54268 | Glucose | −2.26019 | 3.84174 | Methylmalonyl‐CoA | 1.32167 | 3.4627 |
| Gluconic acid | 2.2496 | 4.45531 | Glutamic acid | 1.07994 | 2.85799 | NADP | 1.28716 | 3.32016 |
| Gluconolactone | 2.05367 | 4.49607 | Glyceraldehyde | −2.7688 | 3.51544 | Oxoglutaric acid | −4.48178 | 3.18444 |
| Glucose | 2.61453 | 3.92834 | Guanosine monophosphate | −4.51203 | 3.06703 | Phosphoribosyl pyrophosphate | −2.74597 | 2.4644 |
| Glucose 6‐phosphate | 2.40673 | 4.0459 | Methylmalonyl‐CoA | 1.87291 | 4.16991 | Ribose 1,5‐bisphosphate | −3.25943 | 3.71755 |
| Glutamic acid | 5.45949 | 4.33264 | Oxoglutaric acid | −3.20191 | 3.13281 | Ribose 5‐phosphate | −3.1367 | 4.83038 |
| Glyceraldehyde | 2.21778 | 3.81563 | Phosphoribosyl pyrophosphate | −2.37776 | 2.4204 | Ribulose 5‐phosphate | −3.1367 | 4.83038 |
| Glyceraldehyde 3‐phosphate | 2.21411 | 2.80225 | Propionyl‐CoA | 4.15035 | 2.97379 | Sedoheptulose 7‐phosphate | −1.68031 | 4.5191 |
| Glyceric acid | 1.82435 | 1.53855 | Pyruvic acid | −3.52997 | 6.48381 | Succinyl‐CoA | 1.32167 | 3.4627 |
| Glyceric acid 1,3‐biphosphate | 2.24973 | 1.53222 | Ribose 1,5‐bisphosphate | −2.79329 | 2.99855 | Tyrosine | 1.14909 | 2.01647 |
| Glycine | 2.77207 | 3.11141 | Ribose 5‐phosphate | −1.59255 | 2.6474 | Valine | 1.42203 | 1.81475 |
| Guanosine diphosphate | 1.16527 | 1.88233 | Ribulose 5‐phosphate | −1.59255 | 2.6474 | |||
| Histidine | −3.46801 | 2.38333 | Succinyl‐CoA | 1.87291 | 4.16991 | |||
| Isoleucine | 3.23544 | 2.85877 | Valine | 1.27713 | 3.34286 | |||
| Lysine | 3.24613 | 1.92555 | ||||||
| Malic acid | 2.48498 | 3.51363 | ||||||
| Methionine | 3.7114 | 6.15925 | ||||||
| Methylmalonyl‐CoA | 3.26596 | 2.73429 | ||||||
| Oxoglutaric acid | 3.27512 | 4.91939 | ||||||
| Phosphoribosyl pyrophosphate | −1.3172 | 2.85536 | ||||||
| Proline | 3.2362 | 10.1264 | ||||||
| Propanoyl phosphate | 5.17919 | 1.64887 | ||||||
| Propionic acid | 2.29647 | 2.02239 | ||||||
| Propionyl‐CoA | 6.45464 | 2.72025 | ||||||
| Pyruvic acid | 1.98894 | 3.41543 | ||||||
| Sedoheptulose 7‐phosphate | 1.03178 | 1.73171 | ||||||
| Serine | 4.3842 | 2.44302 | ||||||
| Succinic acid | 3.38252 | 5.73543 | ||||||
| SuccinyCoA | 3.26596 | 2.73429 | ||||||
| Threonine | 1.37103 | 1.32208 | ||||||
| Tryptophan | 5.26622 | 5.09113 | ||||||
| Tyrosine | 4.1815 | 3.85977 | ||||||
| Valine | 3.77485 | 4.81414 | ||||||