Figure 3.

Cell viability tests show cytotoxicity with mitogen-activated protein kinase-dependent recombinant vaccinia virus (MDRVV) alone or in combination with armed MDRVV and 5- fluorocytosine (5-FC) in human pancreatic ductal adenocarcinoma cell lines. (A) Pancreatic cancer cell lines were infected with each recombinant virus at a multiplicity of infection (MOI) of 0.1, and 48 h later. Cells were treated with 0, 1, 10, or 100 μg/mL of 5-FC. Cell viabilities were determined 120 h after virus infection. (B) BxPC-3, PANC-1, and SW 1990 cells were infected with each recombinant virus at an MOI of 0.01 and combined with 5-FC as described in (A). Data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed unpaired t-test).