Figure 3.

Silencing GRK4 reduced growth rate of MDA-MB-468 cells. (A) GRK4 protein levels in the cells expressing GRK4 shRNA 167, empty vector, and non-targeting shRNA; (B) GRK4 mRNA levels in three GRK4 knock down cell lines after doxycycline treatment for 2 weeks; (C) Cell counts. The cells were treated with or without doxycycline (1 µg/ml) for 7 days and then were plated in 6-well plates at the density of 30,000 cells/well. Cell number was counted 7 days later (Total 14-day treatment with doxycycline).