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. 2021 Apr 27;13(5):768. doi: 10.3390/v13050768

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Mosquito species identification by MALDI-TOF MS-based on LSV ranges. (a) Results of MS spectra query for mosquito specimens tested blindly against the MS database were classified according to identification relevance based on LSVs. LSVs > 1.8, reliable identification (green); 1.8 > LSVs > 1.6, identification requiring manual validation (yellow); 1.6 > LSVs; corresponding to unreliable identification and considered as non-identified (red). (b) Representative MS spectra of three mosquito species were collected in this study. Ae. albopictus (A,B), Cx. pipiens (C,D), and Cs. longiareolata (E,F). Spectra analysis was performed using Flex analysis 3.3 software. a.u., arbitrary units; m/z, mass-to-charge ratio.