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. 2021 May 20;20:15347354211017219. doi: 10.1177/15347354211017219

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Effect of TER on Akt/mTOR signaling in effector T-cells. (A and B) The amount of mTOR and phosphorylation of Akt substrates (Ser473 and Thr308) in CD8+ T-cells was detected via Western blot analysis. (C) The expression levels of PD-1 and IFN-γ on CC-CD8+ T-cells were analyzed by flow cytometry. (D) The ﬂow cytometry analysis of IL-10 and IFN-γ production, and the expression of GZMB in CC-CD8+ T-cells with the treatment of TER. Representative results were showed. The percentages of cells were indicated in each quadrant. n = 3 and data was presented as means ± SD.

*P < 0.05, **P < 0.01 compared with the control group.