Figure 6.

Effect of TER on dysfunction in effector T-cells. (A and B) The expression of LDHA and increased expression of IFN-γ in CC-CD8+ T-cells were detected through Western blot analysis. (C) Lactate level in T-cells was assessed. (D) The effect of TER treatment on LDHA was detected by real-time PCR and lactate levels were compared between different groups. (E and F) Transcription and protein expression of IFN-γ and GZMB in CC-CD8+ T-cells were assessed using western blotting, respectively. All the experiments were performed for 3 times. Comparison of lactate levels were performed between the TER treated control IC-CD8+ T-cells and TER treated CC-CD8+ T-cells. n = 3 and data was presented as means ± SD.

*P < 0.05, **P < 0.01 and ***P < 0.001 compared with the control group.