Table A2.

Comparisons for P_app of RAECM-I vs -II with or without EGTA based on three-way ANOVA and post-hoc Tukey’s multiple comparisons. No entry denotes no significance between the two P_app. Comparisons with adjusted p values < 0.05 are listed. **** denotes p < 0.0001, *** denotes p < 0.001, ** denotes p < 0.01 and * denotes p < 0.05.

(a) Comparison table for Papp of RAECM-I vs RAECM-II.
Papp of RAECM-I	Papp of RAECM-II
	Formamide	Acetamide	Ethylene glycol	Glycine	Arabinose	Mannitol	Sucrose	5-Carboxy- Fluorescein	Sulfo- Rhodamine B	FITC-4 kDa Dextran	FITC-10 kDa Dextran
Formamide
Acetamide				**	***	***	***	**	**	**	***
Ethylene glycol
Glycine		****
Arabinose		****	*
Mannitol		***
Sucrose		***
5-Carboxyfluorescein		****	*
Sulforhodamine B		***
FITC-4 kDa dextran		****
FITC-10 kDa dextran		****	*
(b) Comparison table for Papp of RAECM-I vs RAECM-I with EGTA.
Papp of RAECM-I	Papp of RAECM-I with EGTA
	Formamide	Acetamide	Ethylene glycol	Glycine	Arabinose	Mannitol	Sucrose	5-Carboxy- Fluorescein	Sulfo- Rhodamine B	FITC-4 kDa Dextran	FITC-10 kDa Dextran
Formamide
Acetamide
Ethylene glycol
Glycine		***
Arabinose		****
Mannitol		***
Sucrose		***
5-Carboxyfluorescein		****
Sulforhodamine B		***
FITC-4 kDa dextran		****
FITC-10 kDa dextran		****
(c) Comparison table for Papp of RAECM-I vs RAECM-II with EGTA.
Papp of RAECM-I	Papp of RAECM-II with EGTA
	Formamide	Acetamide	Ethylene glycol	Glycine	Arabinose	Mannitol	Sucrose	5-Carboxy- Fluorescein	Sulfo- Rhodamine B	FITC-4 kDa Dextran	FITC-10 kDa Dextran
Formamide	****	****	**	****				****
Acetamide	****	****		****
Ethylene glycol	****	****	**	****
Glycine	****	****	****	****	**
Arabinose	****	****	****		**	*
Mannitol	****	****	****	****	**
Sucrose	****	****	****	****	**
5-Carboxyfluorescein		****	****	****	**	*
Sulforhodamine B	****	****	****	****	**
FITC-4 kDa dextran	****	****	****	****	**	*
FITC-10 kDa dextran	****	****	****	****	**	*
(d) Comparison table for Papp of RAECM-II vs RAECM-I with EGTA.
Papp of RAECM-II	Papp of RAECM-I with EGTA
	Formamide	Acetamide	Ethylene glycol	Glycine	Arabinose	Mannitol	Sucrose	5-Carboxy- Fluorescein	Sulfo- Rhodamine B	FITC-4 kDa Dextran	FITC-10 kDa Dextran
Formamide
Acetamide					**	*	**	*	*	*	*
Ethylene glycol
Glycine		***
Arabinose		****
Mannitol		****
Sucrose		****
5-Carboxyfluorescein		***
Sulforhodamine B		***
FITC-4 kDa dextran		***
FITC-10 kDa dextran		****
(e) Comparison table for Papp of RAECM-II vs RAECM-II with EGTA.
Papp of RAECM-II	Papp of RAECM-II with EGTA
	Formamide	Acetamide	Ethylene glycol	Glycine	Arabinose	Mannitol	Sucrose	5-Carboxy- Fluorescein	Sulfo- Rhodamine B	FITC-4 kDa Dextran	FITC-10 kDa Dextran
Formamide	****	****	*	****				****	****
Acetamide	****	****		****
Ethylene glycol	****	****	*	****
Glycine	****	****	****	****	**
Arabinose	****	****	****		**	*
Mannitol	****	****	****	****	**	*
Sucrose		****	****	****	**	*
5-Carboxyfluorescein		****	****	****	**
Sulforhodamine B		****	****	****	**
FITC-4 kDa dextran	****	****	****	****	**
FITC-10 kDa dextran	****	****	****	****	**	*
(f) Comparison table for Papp of RAECM-I with EGTA vs RAECM-II with EGTA.
Papp of RAECM-I with EGTA	Papp of RAECM-II with EGTA
	Formamide	Acetamide	Ethylene Glycol	Glycine	Arabinose	Mannitol	Sucrose	5-Carboxy- Fluorescein	Sulfo- Rhodamine B	FITC-4 kDa Dextran	FITC-10 kDa Dextran
Formamide	****	****		****
Acetamide	****	****		****
Ethylene glycol	****	****		****
Glycine	****	****	****	****
Arabinose	****	****	****	****
Mannitol	****	****	****	****
Sucrose	****	****	****	****
5-Carboxyfluorescein	****	****	****	****
Sulforhodamine B	****	****	****	****
FITC-4 kDa dextran	****	****	****	****
FITC-10 kDa dextran	****	****	****	****