Table 3.
Effect of polyethylene glycol (PEG) concentration on somatic embryo maturation efficiency of sugi embryogenic cell lines (ECLs). Data are presented as the mean ± SD (standard deviation) of the number of cotyledonary somatic embryos per plate from three replicates for each ECL matured on media containing different concentrations of PEG.
| ECL | Cotyledonary Somatic Embryos per Plate by PEG Concentrations | ||||
|---|---|---|---|---|---|
| PEG 10% | PEG 15% | PEG 17.5% | PEG 20% | Average per Cell Line | |
| S-18 | 222.3 ± 95.6 | 544.0 ± 200.3 | 640.3 ± 61.0 | 36.7 ± 7.8 | 360.8 ± 272.0 a |
| S-100 | 160.3 ± 45.6 | 322.3 ± 109.3 | 532.0 ± 120.0 | 20.0 ± 17.8 | 258.7 ± 211.8 a |
| S-352 | 210.0 ± 100.5 | 395.7 ± 209.9 | 446.3 ± 145.4 | 51.7 ± 19.6 | 275.9 ± 201.2 a |
| T-1151 | 222.3 ± 127.6 | 461.3 ± 77.6 | 812.7 ± 196.2 | 58.7 ± 18.2 | 388.8 ± 314.4 a |
| T-158 | 157.7 ± 73.0 | 317.0 ± 106.2 | 686.0 ± 172.9 | 43.7 ± 25.8 | 301.1 ± 269.7 a |
|
Average per PEG
concentration |
194.5 ± 84.0 b | 408.1 ± 155.4 a | 623.5 ± 180.2 a | 42.1 ± 21.0 c | |
Different letters show significant differences according to Tukey’s post hoc test (p < 0.05).