Table 5.
Effect of abscisic acid (ABA) concentration on somatic embryo maturation efficiency of sugi embryogenic cell lines (ECLs). Data are presented as the mean ± SD (standard deviation) of the number of cotyledonary somatic embryos per plate from three replicates for each ECL matured on media containing different concentrations of ABA.
| ECL | Cotyledonary Somatic Embryos per Plate by ABA Concentrations | ||||
|---|---|---|---|---|---|
| ABA 0 µM | ABA 50 µM | ABA 100 µM | ABA 200 µM | Average per Cell Line | |
| S-18 | 19.3 ± 10.5 | 186.3 ± 161.1 | 385.0 ± 117.4 | 163.7 ± 49.9 | 188.6 ± 161.9 a |
| S-64 | 2.0 ± 1.7 | 273.0 ± 206.9 | 366.0 ± 128.3 | 22.3 ± 8.6 | 165.8 ± 194.4 b |
| S-85 | 2.3 ± 2.5 | 237.7 ± 18.0 | 256.7 ± 64.0 | 344.7 ± 129.6 | 210.3 ± 146.2 b |
| S-100 | 8.7 ± 7.8 | 354.0 ± 62.4 | 377.3 ± 119.4 | 166.0 ± 117.5 | 226.5 ± 174.4 ab |
| S-113 | 1.7 ± 2.1 | 37.3 ± 18.9 | 228.7 ± 113.1 | 77.7 ± 12.0 | 86.3 ± 102.8 b |
| S-352 | 3.3 ± 0.6 | 50.0 ± 25.5 | 331.7 ± 59.5 | 54.3 ± 27.2 | 109.8 ± 138.7 b |
| T-1151 | 8.0 ± 4.6 | 271.7 ± 98.3 | 408.3 ± 205.9 | 139.0 ± 13.5 | 206.8 ± 183.7 ab |
|
Average per ABA
concentration |
6.5 ± 7.5 c | 201.4 ± 145.2 ab | 336.2 ± 122.3 a | 138.2 ± 116.8 b | |
Different letters show significant differences according to Tukey’s post hoc test (p < 0.05).