Table 9.
Effect of amino acid (AA) concentration on somatic embryo maturation efficiency of sugi embryogenic cell lines (ECLs). Data are presented as the mean ± SD (standard deviation) of the number of cotyledonary somatic embryos per plate from three replicates for each ECL matured on media containing different concentrations of AAs.
| ECL | Cotyledonary Somatic Embryos per Plate by AA Concentrations | ||||
|---|---|---|---|---|---|
| 0× | 1× | 2× | 3× | Average per Cell Line | |
| S-18 | 42.0 ± 33.4 | 330.3 ± 211.8 | 452.7 ± 221.2 | 305.0 ± 155.9 | 282.5 ± 214.7 a |
| S-100 | 3.0 ± 2.6 | 156.3 ± 74.9 | 340.7 ± 53.6 | 274.7 ± 39.5 | 193.7 ± 140.7 b |
| S-113 | 6.7 ± 2.5 | 208.7 ± 69.2 | 115.7 ± 38.6 | 94.3 ± 72.2 | 106.3 ± 87.9 b |
| S-182 | 2.3 ± 2.1 | 233.7 ± 83.3 | 274.0 ± 75.6 | 308.0 ± 152.6 | 204.5 ± 148.8 b |
| S-352 | 2.3 ± 2.5 | 268.7 ± 73.7 | 353.3 ± 50.5 | 302.7 ± 110.7 | 231.8 ± 154.3 b |
|
Average per AA
concentration |
11.3 ± 20.5 a | 239.5 ± 115.3 b | 307.3 ± 148.7 b | 256.9 ± 129.4 b | |
Different letters show significant differences according to Tukey’s post hoc test (p < 0.05).