Table 13.
Factor | Best Result | Reference |
---|---|---|
BS in medium | EM | Maruyama et al. 2000 |
PEG concentration | 175 g L−1 | 169-09125 Wako Pure Chemical, Osaka, Japan |
ABA concentration | 100 µM | BIA-0125 Apollo Scientific Ltd., Manchester, UK |
KCl concentration | 0.08 g L−1 | 063-03545 Wako Pure Chemical, Osaka, Japan |
AA concentration | L (+) Glutamine 2 g L−1 L- Asparagine 1 g L−1 L (+) Arginine 0.5 g L−1 |
078-00525 Wako Pure Chemical, Osaka, Japan 013-04815 Wako Pure Chemical, Osaka, Japan 011-04615 Wako Pure Chemical, Osaka, Japan |
PCM | 3-1 (EM proliferation medium) | Maruyama et al. 2000 |