Skip to main content
. 2021 Apr 26;10(5):874. doi: 10.3390/plants10050874

Table 13.

Summary of the optimal factors giving the best results achieved in our six independent experiments including studies on the effect of basal salts (BS) contained in maturation medium, polyethylene glycol (PEG) concentration, abscisic acid (ABA) concentration, additional supplementation of potassium chloride (KCl), amino acid (AA) concentration, and proliferation culture media (PCM), as the main culture factors affecting somatic embryo maturation in sugi.

Factor Best Result Reference
BS in medium EM Maruyama et al. 2000
PEG concentration 175 g L−1 169-09125 Wako Pure Chemical,
Osaka, Japan
ABA concentration 100 µM BIA-0125 Apollo Scientific Ltd.,
Manchester, UK
KCl concentration 0.08 g L−1 063-03545 Wako Pure Chemical,
Osaka, Japan
AA concentration L (+) Glutamine 2 g L−1
L- Asparagine 1 g L−1
L (+) Arginine 0.5 g L−1
078-00525 Wako Pure Chemical,
Osaka, Japan
013-04815 Wako Pure Chemical,
Osaka, Japan
011-04615 Wako Pure Chemical,
Osaka, Japan
PCM 3-1 (EM proliferation medium) Maruyama et al. 2000