Fig. 4.

NCF4 and IL-1 subpathway rare variants and effects on cytokine production. a NCF4 rare variant carriers are in the lowest extreme of PHA-induced residual (corrected for age and sex) IL-6 cytokine production of the histogram distribution. Individuals without NCF4 rare variants are shown in skyblue, individuals carrying a rare variant contributing to the association in red and purple. In b, the variants contributing to the association are annotated in the same red and purple colors on the most abundant transcript of NCF4 (NM_013416). c A combined weighted directional allelic score for IL-1 subpathway rare variants in correlation with LPS-induced residual IL-1β cytokine production that is accompanied by a Spearman R of 0.33, P value = 5.7E⁻¹³