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. 2021 May 25;22:266. doi: 10.1186/s12859-021-04198-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Effects on quantification accuracy. Effect of transcript length on the concordance of each method to the truth, given the average of three liver samples, using A idealized and B realistic data. Effect of number of expressed isoforms (per gene) on the mean |logFC| for both tissues, using C idealized and D realistic data. The maximum number of expressed isoforms (per gene) between the three replicates, and the mean of the |logFC|s at a given number of expressed isoforms (per gene) are plotted. The mean |logFC| is based on at least 100 transcripts