Figure 1.

Computer-controlled bioreactor system with pulsatile flow. (A,B) The bioreactor system consists of computer-controlled regulatory elements and a tube system. (C) Schematic overview of the bioreactor system. Gas exchange occurs by surface aeration in the oxygenation camber (1). pH level (2), oxygen saturation (3), and pressure are measured and maintained via quantity and composition of gas inflow. Temperature (4) is measured and regulated by heating sleeve (5). Perfusion medium is piped through the heart valve by a pulsatile roller pump (6).