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. 2021 Apr 23;11(5):758. doi: 10.3390/diagnostics11050758

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The various pathogenic fungal antigen and GXM capsular polysaccharide antigens were investigated for specificity by indirect ELISA. The absorbance measurement was observed at OD_{450 nm} with error bar represented as mean OD ± SD of triplicated determination. The concentration of GXM was 10 ng/mL. The concentrations of all pathogenic fungal antigens were 10 μg/mL. The cytoplasmic yeast antigens (CYA) of pathogenic fungi were indicated C. neoformans strain H99, serotype A (CN), T. marneffei (TM), C. albicans (CA), C. glabrata (CG), C. krusei (CK), S. schenckii (SS), and Trichosporon sp. (TS). The cytoplasmic mycelial antigens (CMA) were A. fumigatus (AF), P. boydii (PB), and Geotrichum sp. (GE).