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. 2021 Apr 23;11(5):1216. doi: 10.3390/ani11051216

Figure 1.

Figure 1

Fluorescence observation of CPE on LLC-PK1 cells transfected with shRNAs (100×). The LLC-PK1 cells were initially transfected with different plasmids carrying shRNA gene(s) and then infected with PDCoV at an MOI of 0.1, and CPE was observed at 36 hpi. (a,b) LLC-PK1 cells infected with PDCoV. (c–l) LLC-PK1 cells transfected with shRNA-N1, shRNA-N2, shRNA-N3, shRNA-N4, and shRNA-NC, respectively. (m,n) LLC-PK1 cells that were negative controls, that is, neither transfected with plasmids nor infected with virus.