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The reduction of PDCoV RNA and PDCoV N protein triggered by double-shRNA-N1-expressing plasmids. (a) qRT-PCR analysis of the inhibition of viral RNA synthesis in LLC-PK1 cells expressing double-shRNA-N1, the results are marked with three asterisks (***) if p < 0.001; (b) Western blot analysis of double-shRNA-N1-expressing plasmids inhibiting PDCoV N protein expression in LLC-PK1 cells at 36 hpi.
