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. 2021 Apr 27;11(5):396. doi: 10.3390/life11050396

Figure 10.

Figure 10

(a) Left side: Low-field region of the spectra (3–4000 transients) obtained for complexes of human α-thrombin with hirunorm V, hirunorm IV, RGD-hirudin (recombinant 32SGD34 type 2 hirudin), Nα(Me)Arg-peptide and D-NAPAP (Nα-2-naphthylsulfonyl-glycyl-DL-4-amidinophenylalanyl-piperidide acetate) from top to bottom, respectively, in pH 6.5, 0.025–0.050 M citrate buffer, 0.10–0.20 M NaCl, 0.005–0.010 % PEG-8000 at 30 °C. The corresponding peak widths at half height are 91, 82, 43, 38 and 125 Hz. (b) Right side: Temperature dependence of the low-field resonance for the human α-thrombin-hirunorm IV complex in pH 6.5, 0.020 M citrate buffer, 0.10 M NaCl and 0.005% PEG8000. The spectra were recorded at 35, 25, 15, and 5 °C, from top to bottom.