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. 2021 Apr 26;10(5):1017. doi: 10.3390/cells10051017

Figure 2.

Figure 2

LiCl ameliorates the inhibition of myoblast differentiation by CT26 colon carcinoma cell-conditioned media (CCM). (A) Immunofluorescence staining after 72 h of culture in the following media: (1) differentiation media (DM), (2) CCM only, and (3) CCM and 5 mM LiCl (n = 4). (B) Quantified ratio of MyHC-positive nuclei in immunostaining images (n = 4). (C) Representative Western blot images for myosin heavy chain (MyHC), phospho (Ser-9) GSK3β, naïve GSK3β, and alpha-tubulin after 72 h of culture in (1) DM, (2) CCM, (3) CCM and 5 mM LiCl (n = 3). (D) Quantification results of Western blot analysis (n = 3). (E) qPCR analysis of the myotube marker, Myh2, and the myoblast marker, Pax-7, after 24 h of incubation in the following media: (1) GM, (2) DM, (3) CCM, (4) CCM and 5 mM LiCl (n = 5). Statistical significance compared to DM is indicated by * (* = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001). # represents statistical significance compared to the CCM group (# = p-value < 0.05, ## = p-value < 0.01, ### = p-value < 0.001).