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. 2021 May 25;12(6):541. doi: 10.1038/s41419-021-03816-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The typical circular morphology of EVs was observed under electron microscope (EM) from groups C, A, M, and S. B Western blot was used to analyze the EV marker proteins CD9, CD64 and CD81, TSG101, non-EV marker Calnexin and GM130 in groups C, A, M, S, and Cells. Each well was loaded with 20 μg protein. C, D The size distribution curve and distribution proportion of EVs in plasma of C, A, M, and S were shown by NTA analysis. The diameter of EVs in these four groups was mostly concentrated in the interval of 55–145 nm, and the data were shown with mean + SD. There was no significant difference in the proportion of groups C, A, M, and S in each distribution interval (P > 0.05).