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. 2021 Apr 24;10(5):860. doi: 10.3390/plants10050860

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effects of compounds 1–7 on the differentiation of mesenchymal stem cells (MSCs) toward osteoblasts. (A) Stimulatory effect of compounds 1–7 on osteogenic differentiation of MSC. Fully-differentiated C3H10T1/2 cells were stained with alkaline phosphatase (ALP) at 9 d after osteogenic differentiation with 20 µM of compounds 1–7. (B) ALP enzyme activity was measured in osteogenically differentiated C3H10T1/2 cells treated with compounds 1–7. The values were calculated relatively by setting the untreated negative control to 1. Oryzativol A (5 μM) was added to the experimental set as a positive control and marked as OryA. * denotes p < 0.05 and *** denotes p < 0.001.