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. 2021 Apr 24;9(5):465. doi: 10.3390/biomedicines9050465

Figure 1.

Figure 1

DAT hypofunction affects dopaminergic neuromuscular response. (A) Inhibitory concentration–response curves to dopamine (DA) on spontaneous contractile activity of isolated ileal preparations from WT and DAT+/− mice. (B) Representative tracings of responses induced by increasing dopamine (DA) concentration of in WT and DAT+/− preparations. (C) 30 µM dopamine-induced relaxation of CCh-precontracted ileal preparations with or without SCH23390 or sulpiride in isolated ileal segments of WT and DAT+/− mice. (D) Representative confocal microphotographs showing the distribution of D1R (green, marker for D1 receptor) and HuC/D (red, pan-neuronal marker) and (E) D1R density index in LMMP preparations of WT and DAT+/− mice. Scale bars = 22 μm. Data are reported as mean ± SEM. * p < 0.05, *** p < 0.001 vs. WT mice; °° p < 0.01, °°° p < 0.001 vs. respective control in absence of antagonist. n = 5 mice/group.