Table 1.
Characteristics of primary and secondary antibodies and their respective dilutions used for immunohistochemistry on ileal whole-mount preparations.
| Antibody | Host Species | Dilution | Catalog Number | Source |
|---|---|---|---|---|
| Primary Antisera (Clone) | ||||
| HuC/D (16A11) | Mouse biotin-conjugated | 1:100 | A-21272 | Thermo Fisher Scientific (Monza, Italy) |
| nNOS (polyclonal) | Rabbit | 1:100 | 61–700 | Thermo Fisher Scientific |
| GFAP (polyclonal) | Chicken | 1:100 | ab4674 | Abcam (Cambridge, UK) |
| S100β (EP1576Y) | Rabbit | 1:50 | ab52642 | Abcam |
| ChAT (polyclonal) | Goat | 1:100 | AB144P | Sigma-Aldrich (Milan, Italy) |
| D1 receptor (polyclonal) | Rabbit | 1:50 | ab20066 | Abcam |
| Substance P (polyclonal) | Guinea pig | 1:50 | ab10353 | Abcam |
| Secondary Antisera | ||||
| Goat anti-rabbit IgG Alexa 488-conjugated | NA | 1:1000 | A-11008 | Thermo Fisher Scientific |
| Donkey anti-goat IgY Alexa 555-conjiugated | NA | 1:1000 | A-21432 | Thermo Fisher Scientific |
| Goat anti-chicken IgY Alexa 555-conjugated | NA | 1:1000 | A-11039 | Thermo Fisher Scientific |
| Goat anti-guinea pig IgG Alexa Fluor 488-conjugated | NA | 1:1000 | AB_2534117 | Thermo Fisher Scientific |
| Streptavidin Alexa 555-conjugated | NA- | 1:1000 | S21381 | Thermo Fisher Scientific |