Table 1.
Th1:Th2 index for VLPs alone, and for adjuvanted VLPs.
| Groups (n = 6) |
Serum | Intestine | ||
|---|---|---|---|---|
| TG146 | TG146 + CpG | TG146 | TG146 + CpG | |
| IgG1 (Th2) | 0.13 ± 0.002 | 0.16 ± 0.004 | 0.39 ± 0.008 | 0.45 ± 0.005 |
| IgG2a (Th1) | 0.18 ± 0.001 | 0.29 ± 0.003 | 0.55 ± 0.007 | 0.66 ± 0.006 |
| IgG2b (Th1) | 0.11 ± 0.006 | 0.14 ± 0.004 | 0.33 ± 0.002 | 0.45 ± 0.003 |
| Th1:Th2 Index | 1.11 ± 0.004 | 1.32 * ± 0.003 | 1.12 ± 0.003 | 1.23 * ± 0.004 |
The sera and intestines were collected at day 30 post-challenge infection, and the IgG1 and IgG2a antibody responses in both the sera and intestines were determined. The values are optical density readings at 100-fold serum dilution. Index <1 = Th2 polarization. Index >1 = Th1 polarization. Th1 (IgG2a and IgG2b) or Th2 (IgG1) polarization were determined by Th1:Th2 index calculation ([IgG2a + IgG2b]/2)/(IgG1), as previously described [11] (* p < 0.05).