Figure 4.

Platelet activation signatures in whole blood; platelet-monocyte complex (PMC) and platelet-neutrophil (PNC) formation. Citrated whole blood was stimulated with HEPES (to determine baseline levels) or 1 U/ml thrombin. The median fluorescence intensity (MFI) of CD61 (platelet-positive events) on CD45-positive monocytes (a and b) or neutrophils (c and d) were determined by flow cytometry. Healthy controls are shown in the background as the median (line) ± interquartile range (fill) (b and d). Statistical analysis was performed using Wilcoxon matched-pairs signed rank test; ns = not significant, * P < .05, ** P < .01, *** P < .001