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LoD improvement with a 2-step system (a) 1-Step RT-LAMP limit of detection (LoD) confirmed to be at ~500 copies of Twist Biosciences SARS-CoV-2 control as reported by Zhang et al. 2020; (b) 2-step RT/LAMP cartridge with increased sensitivity with LoD down to 25 copies of Twist Biosciences SARS-CoV-2 control.
