Fig. 5. (a) Images of the spheroids stained using the LIVE/DEAD® kit from confocal laser scanning microscopy (CLSM), (scale bar: 75 μm). (b) Viability of MTSs incubated for 24 h, 36 h and 48 h with 11.36 μg mL−1 hydrophobic drug carmofur (HCFU) delivered by different formulations (mean ± SD, n = 5, *p < 0.05). (c) Photographs of A549 MTS treatment with DFMs@HCFU (up) and nDFMs@HCFU (down) on the 1st, 2nd and 4th days (scale bar: 200 μm). (d) A549 MTS growth profiles (4 day-time course) following a single administration of the drug nanocarrier (mean ± SD, n = 15).
