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. 2021 Apr 28;13(5):783. doi: 10.3390/v13050783

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CRISPRi silencing of delayed-early genes in iSLK-219 cells. (A) Region of the viral genome encoding ORF59 and position of the sgRNAs. Immunoblot analysis of (A) ORF59, and (B) K8.1 (in latent and lytic (96 h post-reactivation) in iSLK-219-dC9K cells transduced with a non-targeting (NT) sgRNA or an ORF59-specific sgRNA. Image representative of three biological replicates. Bars: quantification of the data. Error bars: standard deviation (one-way ANOVA, **** p < 0.001, *** p < 0.001). (C) Quantification of viral titers (IU, infectious units) in the filtered supernatant of iSLK-219-dC9K cells transduced with a non-targeting (NT) sgRNA or ORF59-specific sgRNAs, collected at 72 and 96 h post-reactivation. Error bars: standard deviation.