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. 2021 Apr 23;10(5):513. doi: 10.3390/pathogens10050513

Figure 3.

Figure 3

Antibody mapping of PrPres of VPSPr by western blotting. Western blot analysis of PrP in the detergent-insoluble fraction (P2) of the brain homogenates (regular lysis buffer, pH 7.4) of cadavers with VPSPr129MM, VPSPr129MV, or VPSPr129VV after treatment with PK at 50 µg/mL probing with different PrP-specific antibodies. PK-treated PrP in brain homogenates of cadavers with sCJD or non-CJD (CTL) was used as control. Western blotting of PrPres were probed with: (A): 3F4 against PrP106–112 [15,39]; (B): 1E4 against PrP97–105 [16]; (C): Tohoku 2 (T2) antibody against PrP97–103 [37]; (D): EP1802Y against PrP217–226 [38]; (E): anti-C antibody against PrP220–231 [9]; and (F): 9A2 against PrP99–101 [40].