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. 2021 Apr 23;10(5):513. doi: 10.3390/pathogens10050513

Figure 6.

Figure 6

Comparison of electrophoretic profiles of PrP from VPSPr and GSS by western blotting with different PrP-specific antibodies after treatment with different amounts of PK along with or without PNGase F. Brain homogenates from cadavers with GSSP102L or VPSPr129MV were treated with different amounts of PK ranging from 0, 5, 10, 25, and 50 for GSS and 0, 5, 10, 25, 50, 100, and 200 µg/mL for VPSPr129MV at 37 °C for 1 h followed by deglycosylation with PNGase F prior to western blotting probing with different PrP-specific antibodies. (A): 3F4 with PrP epitope 106–112 [15,39]; (B): 1E4 with PrP epitope 97–105 [16]; (C): 9A2 with epitope 99–101 [40]; (D): 12B2 with epitope 90–94 [40]; (E): Anti-C with epitope 220–231 [9].