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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Biochem Pharmacol. 2021 Feb 3;185:114451. doi: 10.1016/j.bcp.2021.114451

Figure 3: SCTR modulators exert PAM activity in NG108–15 cell line:

Figure 3:

cAMP dose-response of (A, B, C, left to right) Sec-FL, Sec(1–23) and Sec(3–27) in NG108 cells treated with DMSO (black), 25 μM of (A) 2-sulfonyl pyrimidines A1 (dark green), A2 (light green), (B) 2-mercapto pyrimidines B1 (light blue), B2 (dark blue) or (C) 2-amino pyrimidines C4 (light red), C9 (dark red); TR-FRET ratios resulting from cAMP accumulation normalized to Sec-FL; graphs plotted using GraphPad Prism; experiments performed in duplicates in at least three independent experiments; data points shown as mean ± SEM. Statistical significance determined using GraphPad Prism’s unpaired t-test corrected with Holm-Sidak method.