Figure 4.

Organoid-forming efficiency within OrganoidChip. (A) Representative images of organoids morphology at days 2 and 6 of culture. On day 6, Iso-50 organoids cultured on-plate exhibited a cystic-like morphology with a visible lumen. In contrast, the organoids cultured on-chip are multi-layered with crypt-like projections or display a thick epithelial cell layer containing glandular structures. Scale bar: 100 µm. (B) Quantification of organoid colony-forming efficiency at day 6 of culture. The results are reported as the mean ± SD of three independent experiments. (C) Analysis of organoid size and area at day 6 of culture. The results are described as mean ± SD of a total of 50 organoids in three independent experiments subjected to the analysis (Student’s t-test: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001).