Figure 1.

Representative density plot for flow cytometric analyses performed on platelet-rich (A) and -poor (B) plasma obtained from a mare susceptible to persistent-breeding induced endometritis. Platelets were identified with a primary (mouse monoclonal antibody anti CD41/61) and secondary antibody anti-mouse IgG conjugated with a fluorochrome (PE, R-phycoerythrin, X-axis) and their membrane integrity was assessed with Zombie Green (Y-axis). The right quadrants enclosed CD41/61 positive events, presumptively corresponding to platelets, with high (damaged membrane) or low (intact membrane) Zombie Green signal. The left quadrants included CD41/61 negative events, likely debris, with high or low Zombie Green signal.