Figure 3.

Kidney histology as diabetes progression. (A) Kidney samples were subjected to histology examination. Hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining analysis of renal histological abnormalities showing hyaline cast of renal tubules, mesangial cell expansion, thickening of the basement membrane, increased mesangial matrix, formation of glomerular hyaline droplet, and accumulation of PAS positive glycogen (black arrow) stores in kidneys. (B) Histology scores for the renal injury were shown. Scores (0, normal; 1, minimal; 2, slight; 3, moderate; 4, marked; 5, severe), ND, normal diet; HFD, high-fat diet; 5 W, 5 weeks; 12 W, 12 weeks. ** p < 0.01, *** p < 0.001.