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Detection of urinary markers in patients with diabetic nephropathy. Urinary excretion levels of CFD, C4b, LIF, CXCR6, NGAL, TIMP-1, and KIM-1 were measured by Western blot analysis. Membrane was stained with Ponceau O solution to visualize the protein. The major band at about 60 kDa was predicted to be albumin.
