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. 2021 May 19;8(3):ENEURO.0547-20.2021. doi: 10.1523/ENEURO.0547-20.2021

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Copyright © 2021 Marks et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — Assessment of inhibitory, postsynaptic gephyrin puncta within the aspinous, proximal dendrites of CA1 pyramidal cells. Biocytin-filled pyramidal cells labeled with an Alexa Fluor 594-conjugated streptavidin probe (red) and gephyrin puncta (green) were analyzed. A, B, B’, A third channel (blue, with darker blue puncta are in front of the dendrite; lighter blue puncta are behind the dendrite; B, B’), only identifying the gephyrin puncta that were adjacent or overlapped with the aspinous portions of the basilar (C) or perisomatic apical (D) pyramidal cell dendrite, from 3D-reconstructed images was quantified. No changes in the number of gephyrin-immunoreactive puncta were observed. Box Dimensions in A: 100 × 100 × 20 μm. Data represent the mean number of gephyrin-positive puncta ± SEM per 10-μm length of dendrite.