Table 2.

Plasma metabolic measures in female offspring at birth and 19 weeks post-weaning.

	1X						5X
	1X-FA			1X-MTHF			5X-FA			5X-MTHF			Two-way ANOVA p-Value
	Mean	±	S.E.M.	Mean	±	S.E.M.	Mean	±	S.E.M.	Mean	±	S.E.M.	Dose	Form	Dose × Form
Birth
Leptin ng/mL	0.86	±	0.25	1.32	±	0.28	1.93	±	0.28	1.64	±	0.37	0.03	0.78	0.23
Insulin ng/mL	0.30	±	0.05	0.19	±	0.05	0.35	±	0.05	0.29	±	0.08	0.20	0.17	0.68
Glucose mg/dL	74.38	±	6.39	70.30	±	7.83	77.30	±	7.24	72.05	±	9.58	0.77	0.56	0.94
HOMA-IR	0.22	±	0.05	0.15	±	0.07	0.29	±	0.06	0.21	±	0.07	0.34	0.27	0.97
19 weeks PW
Leptin ng/mL	14.08	±	2.12	11.15	±	2.12	17.18	±	1.95	18.35	±	2.12	0.02	0.67	0.33
Leptin/VAT	0.29	±	0.05	0.27	±	0.04	0.40	±	0.04	0.37	±	0.04	0.02	0.53	0.85
Ghrelin ng/mL	153.24	±	18.23	110.19	±	19.21	110.19	±	17.38	102.79	±	19.21	0.37	0.08	0.16
Insulin ng/mL	2.00	±	0.24	1.76	±	0.22	2.25	±	0.26	1.95	±	0.22	0.62	0.26	0.91
Glucose mg/dL	120.57	±	7.11	115.81	±	6.71	129.40	±	7.60	127.67	±	6.36	0.15	0.64	0.83
HOMA-IR	2.53	±	0.38	2.54	±	0.36	3.05	±	0.41	2.62	±	0.34	0.43	0.58	0.56
TG nmol/dL	70.01	±	7.88	83.08	±	7.37	45.17	±	7.88	70.08	±	7.88	0.02	0.02	0.45

Values are least square Mean ± standard error of the mean (S.E.M.), n = 8–10/group. Analyzed by two-way ANOVA with Dose (1X or 5X) and Form (FA vs. MTHF) as main factors and a Dose×Form interaction term. Significant at p < 0.05. Values in bold indicate significant effects. Abbreviations: AIN-93G rodent diet with either 1X-FA, recommended folic acid (2 mg/kg diet); 5X-FA (10 mg/kg diet); 1X-MTHF, equimolar recommended [6S]-5-methyltetrahydrofolic acid calcium salt (2.1 mg/kg diet); or 5X-MTHF (10.4 mg/kg diet); VAT, visceral adipose tissue; HOMA-IR, homeostatic model assessment insulin resistance calculated as follows: [fasting glucose (in mg/dL) fasting insulin (in U/mL)]/2430]; TG, triglycerides; PW, post-weaning.