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. 2021 Apr 28;9(5):482. doi: 10.3390/biomedicines9050482

Figure 4.

Figure 4

γ-secretase inhibitor, DAPT, altered the celastrol and triptolide-induced suppression of notch signaling in TNBC cells. (A) Western blot analysis of BT20 cells treated with DAPT, celastrol, and triptolide for 48 h. NICD1 and HES1 protein expression were suppressed in celastrol and triptolide treatment and further decreased in combination with DAPT. (B) Cell proliferation was significantly inhibited following treatment with the compounds and further reduced in combination of DAPT. Each value represents mean ± SD of four separate estimations, n = 3. * p < 0.05 when compared with control; # p < 0.05 when compared with DAPT control. (C,D) DAPT treatment alone reduces the mammosphere formation when compared to the control, and a combination with celastrol and triptolide shows further reduction in mammosphere growth. Each value represents mean ± SD of four separate estimations, n = 3. * p < 0.05 when compared with control; # p < 0.05 when compared with DAPT control. (E) Expression of stem cell marker proteins DCLK1 and ALDH1 is reduced following DAPT treatment. Combination treatment with celastrol or triptolide further decreases the expression of these proteins in mammospheres.